Saudi Journal of Kidney Diseases and Transplantation

ORIGINAL ARTICLE
Year
: 2011  |  Volume : 22  |  Issue : 3  |  Page : 501--510

Does injection of metanephric mesenchymal cells improve renal function in rats?


Yu-qing Jiao, Zhu-wen Yi, Xiao-jie He, Xi-hong Liu, Qing-nan He, Dan-lin Huang 
 Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China

Correspondence Address:
Zhu-wen Yi
Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011
China

Chronic kidney disease (CKD) is a massive global health-care problem. Cell therapy offers a potential treatment for CKD. The aim of this study was to investigate whether the administration of a population of stem cells could be used to treat adriamycin (ADR)-induced glomerulopathy in rats, a form of CKD. We intravenously transplanted metanephric mesenchymal cells (MMCs) into rats treated with ADR. We also induced MMC differentiation in vitro using a medium derived from serum and homogenates of ADR-induced glomerulopathy rats. We detected the induction of an early epithelial phenotype (cytokeratin-18 expression) and a proximal tubule phenotype (vitamin D receptor expression) in vitro, and MMC-derived epithelial cells corresponding to the proximal tubule and glomeruli in vivo. Transplantation of MMCs after induction of glomerulopathy significantly increased the creatinine clearance rate (Ccr), a marker for glomerular filtration rate, but had no significant effect on other parameters (24-hour urinary protein excretion, serum albumin, total cholesterol). In addition, there was no significant difference in blood urea nitrogen or serum creatinine levels in rats with and without ADR administration. Our results indicate that MMCs might survive, engraft and differentiate into renal epithelia in vivo when transplanted into ADR-treated rats. However, further studies are needed to determine whether MMC transplantation improves renal function and causes renal repair in this model.


How to cite this article:
Jiao Yq, Yi Zw, He Xj, Liu Xh, He Qn, Huang Dl. Does injection of metanephric mesenchymal cells improve renal function in rats?.Saudi J Kidney Dis Transpl 2011;22:501-510


How to cite this URL:
Jiao Yq, Yi Zw, He Xj, Liu Xh, He Qn, Huang Dl. Does injection of metanephric mesenchymal cells improve renal function in rats?. Saudi J Kidney Dis Transpl [serial online] 2011 [cited 2023 Jan 28 ];22:501-510
Available from: https://www.sjkdt.org/article.asp?issn=1319-2442;year=2011;volume=22;issue=3;spage=501;epage=510;aulast=Jiao;type=0